Identification and characterization of guanosine 5′-monophosphate reductase of Trypanosoma Congolese as a drug target.

dc.contributor.authorMusinguzi, Simon Peter
dc.contributor.authorEka, Albertus
dc.contributor.authorSarwono, Yudistira
dc.contributor.authorSuganuma, Keisuke
dc.contributor.authorMitsuhashi, Shinya
dc.contributor.authorOkada, Tadashi
dc.contributor.authorShigetomi, Kengo
dc.contributor.authorNoboru, Inoue
dc.contributor.authorUbukata, Makoto
dc.date.accessioned2018-12-20T10:41:17Z
dc.date.available2018-12-20T10:41:17Z
dc.date.issued2017
dc.description.abstractTrypanosoma Congolese is one of the most prevalent pathogens which causes trypanosomosis in African animals, resulting in a significant economic loss. In its life cycle, T. Congolese is incapable of synthesizing purine nucleotides via a de novo pathway, and thus relies on a salvage pathway to survive. In this study, we identified a gene from T. Congolese, TcIL3000_5_1940, as a guanosine 5′-monophosphate reductase (GMPR), an enzyme that modulates the concentration of intracellular guanosine in the pathogen. The recombinant protein was expressed in Escherichia coli, and the gene product was enzymatically confirmed as a unique GMPR, designated as rTcGMPR. This enzyme was constitutively expressed in glycosomes at all of the parasite's developmental stages similar to other purine nucleotide metabolic enzymes. Mycophenolic acid (MPA) was found to inhibit rTcGMPR activity. Hence, it is a potential lead compound for the design of trypanocidal agents, specifically GMPR inhibitor.en_US
dc.identifier.citationCitation: Musinguzi, S. P., Sarwono, A. E. Y., Suganuma, K., Mitsuhashi, S., Okada, T., Shigetomi, K., Inoue, N. and Ubukata, M., 2017. Identification and characterization of guanosine 5′-monophosphate reductase of Trypanosoma Congolese as a drug target. Parasitology international.en_US
dc.identifier.urihttp://hdl.handle.net/20.500.12280/1281
dc.language.isoenen_US
dc.publisherParasitology internationalen_US
dc.subjectAfrican trypanosomosisen_US
dc.subjectGMP reductaseen_US
dc.subjectPurine metabolic pathwayen_US
dc.subjectTrypanosoma Congoleseen_US
dc.titleIdentification and characterization of guanosine 5′-monophosphate reductase of Trypanosoma Congolese as a drug target.en_US
dc.typeArticleen_US

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